PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We present a polarized light-sheet microscopy and tomography system, PLμTo, for versatile multi-scale and multi-modal volumetric optical imaging. The system can utilize fluorescence and elastic scattering contrast for visualization of biochemical and structural information. PLμTo is compatible with traditional slide and well-plate sample mounting, as well as microfluidics for high-throughput volumetric imaging flow cytometry of mesenchymal stem cells cultured on spherical microcarriers in a bioreactor culture. Additionally, the system is capable of rapidly screening millimeter-sized cleared tissues with cellular level resolution in a few minutes. This system can be utilized to study biological processes at varying spatial and temporal scales.
Oscar R. Benavides andAlex J. Walsh
"PLμTO: a polarized light-sheet microscopy and tomography system for versatile multi-scale and high-throughput cellular resolution imaging", Proc. SPIE PC12827, Multiscale Imaging and Spectroscopy V, PC128270Q (13 March 2024); https://doi.org/10.1117/12.3001188
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Oscar R. Benavides, Alex J. Walsh, "PLUTO: a polarized light-sheet microscopy and tomography system for versatile multi-scale and high-throughput cellular resolution imaging," Proc. SPIE PC12827, Multiscale Imaging and Spectroscopy V, PC128270Q (13 March 2024); https://doi.org/10.1117/12.3001188