Multiscale OCT imaging for high-throughput assessment of oocytes and embryos

Julien Camard; Carla Canedo-Ribeiro; Giuseppe Silvestri; Manuel Jorge Marques; Fatima Acosta; Estefania Hernandez; Gerardo Mendizabal-Ruiz; Darren Griffin; Alejandro Chavez-Badiola; Adrian Podoleanu

doi:10.1117/12.3005696

13 March 2024 Multiscale OCT imaging for high-throughput assessment of oocytes and embryos

Julien Camard, Carla Canedo-Ribeiro, Giuseppe Silvestri, Manuel Jorge Marques, Fatima Acosta, Estefania Hernandez, Gerardo Mendizabal-Ruiz, Darren Griffin, Alejandro Chavez-Badiola, Adrian Podoleanu

Author Affiliations +

Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC1283024 (2024) https://doi.org/10.1117/12.3005696
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present a multiscale imaging system in which a conventional microscope stand is inserted in the sample arm of a Spectral-Domain Optical Coherence Microscopy (SD-OCM) system. The instrument was designed to facilitate localisation and maturity assessment of oocytes and early-stage embryos to be used for in vitro fertilisation (IVF), by a versatile and easy switch of microscope objectives. The dispersion variation due to change of the microscope objectives is dealt with by employing the Complex Master Slave procedure that enables correct operation without the use of matching glass in the reference arm for dispersion compensation.

Conference Presentation

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Julien Camard, Carla Canedo-Ribeiro, Giuseppe Silvestri, Manuel Jorge Marques, Fatima Acosta, Estefania Hernandez, Gerardo Mendizabal-Ruiz, Darren Griffin, Alejandro Chavez-Badiola, and Adrian Podoleanu "Multiscale OCT imaging for high-throughput assessment of oocytes and embryos", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC1283024 (13 March 2024); https://doi.org/10.1117/12.3005696

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