Advanced multi-dimensional SIM: aberration-free, high-speed 3D, multi-color, and deep learning based virtual multi-channel Imaging.

Daozheng Gong; Chufan Cai; Ashesh Ashesh; Geneva Schlafly; Patrick J. La Rivière; Florian Jug; Jing Chen; Norbert F. Scherer

doi:10.1117/12.3017721

13 March 2024 Advanced multi-dimensional SIM: aberration-free, high-speed 3D, multi-color, and deep learning based virtual multi-channel Imaging.

Daozheng Gong, Chufan Cai, Ashesh Ashesh, Geneva Schlafly, Patrick J. La Rivière, Florian Jug, Jing Chen, Norbert F. Scherer

Author Affiliations +

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480N (2024) https://doi.org/10.1117/12.3017721
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

We have devised an innovative SIM imaging system to addresses these issues and achieves the following: (1) Aberration free super-Resolution Imaging: We introduce a hybrid adaptive optics system, TACO, correcting both illumination and excitation aberrations. This results in high-quality SIM super-resolution imaging even in highly aberrative environments. (2) Scalable multicolor Imaging: A dispersion compensation grating module was designed to correct the divergence of different excitation wavelength beams, enabling effortless increasement of color channels number with minimal realignment. (3) Multiplane 3D Imaging: Simultaneous multiplane imaging is achieved through a specialized multi-plane beam splitter. A deformable mirror facilitates rapid z-scan and aberration correction, ensuring fast, aberration-free volumetric imaging. (4) Automated Structure Decomposition: Our system adapts a convolutional neural network model with a novel architecture for automated decomposition of multiple cellular structures in a single channel. This innovation significantly broadens the application of SIM in complex high-dimensional data acquisition tasks.

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Daozheng Gong, Chufan Cai, Ashesh Ashesh, Geneva Schlafly, Patrick J. La Rivière, Florian Jug, Jing Chen, and Norbert F. Scherer "Advanced multi-dimensional SIM: aberration-free, high-speed 3D, multi-color, and deep learning based virtual multi-channel Imaging.", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480N (13 March 2024); https://doi.org/10.1117/12.3017721

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