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13 March 2024 In vivo flow cytometry: counting blood cells without drawing blood
Charles P. Lin
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Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530I (2024) https://doi.org/10.1117/12.3008165
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
The technique of in vivo flow cytometry was developed for the detection and quantification of fluorescent cells in blood circulation without the need for drawing blood samples. In its simplest form, a stationary excitation slit was focused onto a blood vessel and the fluorescence of individual cells was detected through a confocal slit. It has become a very useful tool for noninvasive analysis of circulating leukocytes or tumor cells in animal models where the cell populations of interest can be fluorescently tagged. Translation to human use, however, requires a new label-free approach, which will be discussed in this presentation.
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Charles P. Lin "In vivo flow cytometry: counting blood cells without drawing blood", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530I (13 March 2024); https://doi.org/10.1117/12.3008165
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KEYWORDS
Blood
Flow cytometry
In vivo imaging
Design and modelling
Biological imaging
Image analysis
White blood cells
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