Robust supercontinuum generation using an yttrium aluminum garnet crystal for multimodal label-free multiphoton microscopy

Alejandro De la Cadena; Jaena Park; Kayvan Forouhesh Tehrani; Carlos A. Renteria; Guillermo Monroy; Stephen A. Boppart

doi:10.1117/12.3002654

13 March 2024 Robust supercontinuum generation using an yttrium aluminum garnet crystal for multimodal label-free multiphoton microscopy

Alejandro De la Cadena, Jaena Park, Kayvan Forouhesh Tehrani, Carlos A. Renteria, Guillermo Monroy, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540N (2024) https://doi.org/10.1117/12.3002654
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Nonlinear microscopy encompasses a range of imaging techniques that leverage laser technology to reveal the chemical composition and structure of a sample. Nonlinear microscopes exploit femtosecond laser pulses to target intrinsic biomolecules of cells and tissues. Fiber lasers have limited bandwidth and reduced wavelength tunability, leading to long pulse durations and limited molecular applications. Supercontinuum generation solves this problem, enabling <50 fs pulses and a larger range of molecular excitation. This paper presents nonlinear microscopy with supercontinuum generation from a Yttrium Aluminum Garnet (YAG) crystal enabling simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy with high resolution and specificity in biological tissues.

Conference Presentation

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Alejandro De la Cadena, Jaena Park, Kayvan Forouhesh Tehrani, Carlos A. Renteria, Guillermo Monroy, and Stephen A. Boppart "Robust supercontinuum generation using an yttrium aluminum garnet crystal for multimodal label-free multiphoton microscopy", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540N (13 March 2024); https://doi.org/10.1117/12.3002654

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