Multispectral functional imaging of organoids and 3D cell cultures

Lev T. Perelman

doi:10.1117/12.3011297

13 March 2024 Multispectral functional imaging of organoids and 3D cell cultures

Lev T. Perelman

Proceedings Volume PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; PC128620M (2024) https://doi.org/10.1117/12.3011297
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Stem cell-derived organoids which are miniature self-organized three-dimensional tissue cultures could be a limitless source of functional tissue for transplantations in many organs and also have the potential to significantly improve the drug discovery process. One common feature to all organoids is that they are generated from either pluripotent stem cells (PSCs) or adult stem cells through replicating human development or organ regeneration in vitro. A key challenge with developing any organoid model is monitoring the maturation process or the response of organoids to changes in environment. Here, we will show that a combination of chromatin-sensitive coherent confocal light absorption and scattering spectroscopic (C-CLASS) microscopy and Raman microspectroscopy, which is sensitive to the vibrational modes of molecules and enables direct classification of a broad range of biomolecules with spectral unmixing, provides an efficient way of functional analysis of organoids and 3D cell cultures without the need to sacrifice a sample.

Conference Presentation

Citation Download Citation

Lev T. Perelman "Multispectral functional imaging of organoids and 3D cell cultures", Proc. SPIE PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, PC128620M (13 March 2024); https://doi.org/10.1117/12.3011297

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