Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear

Leonardo Sacconi; Iva Marija Tolic-Nørrelykke; Renzo Antolini; Francesco S. Pavone

doi:10.1117/1.1854675

1 January 2005 Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear

Leonardo Sacconi, Iva Marija Tolic-Nørrelykke, Renzo Antolini, Francesco S. Pavone

Author Affiliations +

Journal of Biomedical Optics, Vol. 10, Issue 1, 014002 (January 2005). https://doi.org/10.1117/1.1854675

Abstract

We use near-IR femtosecond laser pulses for a combination of microscopy and nanosurgery on fluorescently labeled structures within living cells. Three-dimensional reconstructions of microtubule structures tagged with green fluorescent protein (GFP) are made during different phases of the cell cycle. Further, the microtubules are dissected using the same laser beam but with a higher laser power than for microscopy. We establish the viability of this technique for the cells of a fission yeast, which is a common model to study the mechanics of cell division. We show that nanosurgery can be performed with submicrometer precision and without visible collateral damage to the cell. The energy is primarily absorbed by the GFP molecules, and not by other native structures in the cell. GFP is particularly suitable for multiphoton excitation, as its excitation wavelength near 900 nm is benign for most cellular structures. The ability to use GFP to label structures for destruction by multiphoton excitation may be a valuable tool in cell biology.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Leonardo Sacconi, Iva Marija Tolic-Nørrelykke, Renzo Antolini, and Francesco S. Pavone "Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear," Journal of Biomedical Optics 10(1), 014002 (1 January 2005). https://doi.org/10.1117/1.1854675

Published: 1 January 2005

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 98 scholarly publications.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Spindles

Green fluorescent protein

3D image processing

Microscopy

Absorption

Yeast

Image segmentation

Show All Keywords

RELATED CONTENT

Investigating antibiotics in cells with pre resonance stimulated Raman scattering...
Proceedings of SPIE (December 03 2021)

Three dimensional photoacoustic imaging and inversion for accurate quantification of...
Proceedings of SPIE (March 03 2017)

Analysis of somitogenesis using multiphoton laser scanning microscopy (MPLSM)
Proceedings of SPIE (April 24 2001)

Laser nanosurgery and manipulation in living cells
Proceedings of SPIE (March 29 2005)

Optical micromanipulation inside the cell a focus in cell...
Proceedings of SPIE (February 22 2006)

Third-harmonic generation (THG) imaging: 3D contrast without absorption
Proceedings of SPIE (May 06 1999)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years