Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements

Michael Gösch; Hans Blom; Sylvain Anderegg; Kerstin Korn; Per Thyberg; Mona Wells; Theo Lasser; Rudolf Rigler; Anders Magnusson; Sverker Hard

doi:10.1117/1.2080707

1 September 2005 Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements

Michael Gösch, Hans Blom, Sylvain Anderegg, Kerstin Korn, Per Thyberg, Mona Wells, Theo Lasser, Rudolf Rigler, Anders Magnusson, Sverker Hard

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Journal of Biomedical Optics, Vol. 10, Issue 5, 054008 (September 2005). https://doi.org/10.1117/1.2080707

Abstract

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2×2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2×2 fiber arrays.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Michael Gösch, Hans Blom, Sylvain Anderegg, Kerstin Korn, Per Thyberg, Mona Wells, Theo Lasser, Rudolf Rigler, Anders Magnusson, and Sverker Hard "Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements," Journal of Biomedical Optics 10(5), 054008 (1 September 2005). https://doi.org/10.1117/1.2080707

Published: 1 September 2005

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KEYWORDS

Molecules

Luminescence

Diffractive optical elements

Spectroscopy

Fluorescence spectroscopy

Biological research

Bandpass filters

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