Bioenzymatic detection of troponin C using micro-opto-electro-mechanical systems

Amritsar Jeetender; Ion G. Stiharu; Packirisamy Muthukumaran

doi:10.1117/1.2186326

1 March 2006 Bioenzymatic detection of troponin C using micro-opto-electro-mechanical systems

Amritsar Jeetender, Ion G. Stiharu, Packirisamy Muthukumaran

Author Affiliations +

Journal of Biomedical Optics, Vol. 11, Issue 2, 021010 (March 2006). https://doi.org/10.1117/1.2186326

Abstract

Diagnosis and monitoring of critical diseases such as acute myocardial infarction (AMI) require a quantitative analysis of biological molecules. A high-throughput identification of these biological molecules can be generated by using micro-electro-mechanical systems (MEMS) structures like simple cantilever beams, which respond to the intermolecular forces resulting from binding these molecules. Biochemical markers like troponin C are considered the primary markers for myocardial injury and have generated considerable interest. A 26-residue lytic membrane protein of bee venom melittin (ME) is chosen to interact with rabbit skeletal muscle troponin C (TnC) on the surface of the cantilever beams. An optical beam deflection method is employed to identify the enzymatic reaction on the surface of the cantilever due to these proteins. Identification of these proteins is also done using fluorescence spectroscopy (FS) to compliment the optical monitored deflection method. A second set of proteins like horse raddish peroxide (HRP) and hydrogen peroxide (H₂O₂) are applied to atomic force microscopy (AFM) cantilever beams to study their behavior under the enzymatic reactions of proteins. Identification of these proteins is done using Fourier transform infrared spectroscopy (FTIR). An analytical model of the cantilever beam is developed, and its mode shapes are studied by employing orthogonal polynomials in the classic Rayleigh-Ritz method. The surface stress caused by the enzymatic reaction of the proteins that leads to pure bending on the top surface of the cantilever is evaluated. The information provided by the experimental and analytical modeling reported in this work will be useful in the development of a portable biosensor for the detection of AMI.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Amritsar Jeetender, Ion G. Stiharu, and Packirisamy Muthukumaran "Bioenzymatic detection of troponin C using micro-opto-electro-mechanical systems," Journal of Biomedical Optics 11(2), 021010 (1 March 2006). https://doi.org/10.1117/1.2186326

Published: 1 March 2006

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KEYWORDS

Proteins

FT-IR spectroscopy

Molecules

Fluorescence spectroscopy

Calcium

Beam shaping

Biological research

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