Automatic identification of biological microorganisms using three-dimensional complex morphology

Seokwon Yeom; Bahram Javidi

doi:10.1117/1.2187017

1 March 2006 Automatic identification of biological microorganisms using three-dimensional complex morphology

Seokwon Yeom, Bahram Javidi

Author Affiliations +

Journal of Biomedical Optics, Vol. 11, Issue 2, 024017 (March 2006). https://doi.org/10.1117/1.2187017

Abstract

We propose automated identification of microorganisms using three-dimensional (3-D) complex morphology. This 3-D complex morphology pattern includes the complex amplitude (magnitude and phase) of computationally reconstructed holographic images at arbitrary depths. Microscope-based single-exposure on-line (SEOL) digital holography records and reconstructs holographic images of the biological microorganisms. The 3-D automatic recognition is processed by segmentation, feature extraction by Gabor-based wavelets, automatic feature vector selection by graph matching, training rules, and a decision process. Graph matching combined with Gabor feature vectors measures the similarity of complex geometrical shapes between a reference microorganism and unknown biological samples. Automatic selection of the training data is proposed to achieve a fully automatic recognition system. Preliminary experimental results are presented for 3-D image recognition of Sphacelaria alga and Tribonema aequale alga.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Seokwon Yeom and Bahram Javidi "Automatic identification of biological microorganisms using three-dimensional complex morphology," Journal of Biomedical Optics 11(2), 024017 (1 March 2006). https://doi.org/10.1117/1.2187017

Published: 1 March 2006

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 13 scholarly publications and 6 patents.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microorganisms

3D image processing

Holograms

Digital holography

3D image reconstruction

Image segmentation

Holography

Show All Keywords

RELATED CONTENT

Processing of digital holograms: segmentation and inpainting
Proceedings of SPIE (October 31 2016)

Review on edge extraction as pre processing in optical scanning...
Proceedings of SPIE (October 09 2021)

Image processing of real world three dimensional objects sensed with...
Proceedings of SPIE (June 05 2008)

Bio-holographic image segmentation by using interactive graph-cut
Proceedings of SPIE (October 15 2012)

Three-dimensional imaging and recognition of microorganisms using computational holography
Proceedings of SPIE (May 19 2006)

3D imaging, visualization, and recognition of biological micro-organisms
Proceedings of SPIE (October 17 2006)

Segmentation of three-dimensional scenes encoded in digital holograms
Proceedings of SPIE (September 03 2008)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years