Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells

Steven Boutrus; Cherry Anne Greiner; Derrick Hwu; Michael Chan; Charlotte Kuperwasser; Charles P. Lin; Irene Georgakoudi

doi:10.1117/1.2722733

1 March 2007 Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells

Steven Boutrus, Cherry Anne Greiner, Derrick Hwu, Michael Chan, Charlotte Kuperwasser, Charles P. Lin, Irene Georgakoudi

Author Affiliations +

Journal of Biomedical Optics, Vol. 12, Issue 2, 020507 (March 2007). https://doi.org/10.1117/1.2722733

Abstract

The recent introduction of the in vivo flow cytometer for real-time, noninvasive detection and quantification of cells circulating in the vasculature of small animals has provided a powerful tool for tracking the roles of different types of cells in disease progression. We describe a portable version of the device, which provides the capability to: a) excite and detect fluorescence at two distinct colors simultaneously, and b) perform data analysis in real time. These advances improve significantly the utility of the instrument and provide a means of increasing detection specificity. As examples, we present the depletion kinetics of circulating green fluorescent protein (GFP)-labeled breast cancer cells in the vasculature of mice, and the specific detection of circulating hematopoietic stem cells labeled in vivo with two antibodies

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Steven Boutrus, Cherry Anne Greiner, Derrick Hwu, Michael Chan, Charlotte Kuperwasser, Charles P. Lin, and Irene Georgakoudi "Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells," Journal of Biomedical Optics 12(2), 020507 (1 March 2007). https://doi.org/10.1117/1.2722733

Published: 1 March 2007

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY