Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography

Mark Oldham; Harshad Sakhalkar; Tim Oliver; G. Allan Johnson; Mark W. Dewhirst D.V.M.

doi:10.1117/1.2907968

1 March 2008 Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography

Mark Oldham, Harshad Sakhalkar, Tim Oliver, G. Allan Johnson, Mark W. Dewhirst D.V.M.

Author Affiliations +

Journal of Biomedical Optics, Vol. 13, Issue 2, 021113 (March 2008). https://doi.org/10.1117/1.2907968

Abstract

Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8 cm³. A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible "fluorescence-friendly" optical clearing protocols.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Mark Oldham, Harshad Sakhalkar, Tim Oliver, G. Allan Johnson, and Mark W. Dewhirst D.V.M. "Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography," Journal of Biomedical Optics 13(2), 021113 (1 March 2008). https://doi.org/10.1117/1.2907968

Published: 1 March 2008

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KEYWORDS

Tumors

Tissue optics

3D image processing

Optical clearing

3D image reconstruction

Biomedical optics

Tissues

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