Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats

Seonkyung Lee; Danthu H. Vu; Michael F. Hinds; Steven J. Davis; Alvin Liang; Tayyaba Hasan

doi:10.1117/1.3042265

1 November 2008 Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats

Seonkyung Lee, Danthu H. Vu, Michael F. Hinds, Steven J. Davis, Alvin Liang, Tayyaba Hasan

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Journal of Biomedical Optics, Vol. 13, Issue 6, 064035 (November 2008). https://doi.org/10.1117/1.3042265

Abstract

Photodynamic therapy (PDT) is a promising cancer treatment that involves optical excitation of photosensitizers that promote oxygen molecules to the metastable O₂(a¹Δ) state (singlet oxygen). This species is believed to be responsible for the destruction of cancerous cells during PDT. We describe a fiber optic-coupled, pulsed diode laser-based diagnostic for singlet oxygen. We use both temporal and spectral filtering to enhance the detection of the weak O₂(a→X) emission near 1.27 µm. We present data that demonstrate real-time singlet oxygen production in tumor-laden rats with chlorin e6 and 5-aminolevulinic acid-induced protoporphyrin photosensitizers. We also observe a positive correlation between post-PDT treatment regression of the tumors and the relative amount of singlet oxygen measured. These results are promising for the development of the sensor as a real-time dosimeter for PDT.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Seonkyung Lee, Danthu H. Vu, Michael F. Hinds, Steven J. Davis, Alvin Liang, and Tayyaba Hasan "Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats," Journal of Biomedical Optics 13(6), 064035 (1 November 2008). https://doi.org/10.1117/1.3042265

Published: 1 November 2008

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