Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo

Junjie Yao; Konstantin I. Maslov; Song Hu; Lihong V. Wang

doi:10.1117/1.3251044

1 September 2009 Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo

Junjie Yao, Konstantin I. Maslov, Song Hu, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 14, Issue 5, 054049 (September 2009). https://doi.org/10.1117/1.3251044

Abstract

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks-especially capillaries-are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Junjie Yao, Konstantin I. Maslov, Song Hu, and Lihong V. Wang "Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo," Journal of Biomedical Optics 14(5), 054049 (1 September 2009). https://doi.org/10.1117/1.3251044

Published: 1 September 2009

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