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1 July 2010 Fluorescence imaging of membrane dynamics in living cells
Petra Weber, Michael Wagner, Herbert Schneckenburger
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Journal of Biomedical Optics, Vol. 15, Issue 4, 046017 (July 2010). https://doi.org/10.1117/1.3470446
Abstract
Methods of wide-field fluorescence microscopy for measuring membrane dynamics of living cells are described, including spectral imaging as well as anisotropy imaging of the membrane marker 6-dodecanoyl-2-dimethylamino naphthalene (laurdan). Plasma membranes are selected by illumination with an evanescent electromagnetic field and distinguished from intracellular membranes assessed by whole-cell illumination. While fluorescence spectra of laurdan appeared red-shifted with decreasing membrane stiffness, fluorescence anisotropy and rotational correlation times were reduced with increasing membrane fluidity. Membrane stiffness was found to increase with decreasing temperature and increasing amounts of cholesterol and was always higher for the plasma membrane than for intracellular membranes. These effects may have some clinical relevance in the research of drug resistance or cell aging.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Petra Weber, Michael Wagner, and Herbert Schneckenburger "Fluorescence imaging of membrane dynamics in living cells," Journal of Biomedical Optics 15(4), 046017 (1 July 2010). https://doi.org/10.1117/1.3470446
Published: 1 July 2010
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Cited by 27 scholarly publications.
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KEYWORDS
Luminescence
Plasma
Fluorescence anisotropy
Anisotropy
Polarization
Cameras
Microscopy
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