Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission

Arijit K. De; Debjit Roy; Debabrata Goswami

doi:10.1117/1.3645082

1 October 2011 Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission

Arijit K. De, Debjit Roy, Debabrata Goswami

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Journal of Biomedical Optics, Vol. 16, Issue 10, 100505 (October 2011). https://doi.org/10.1117/1.3645082

Abstract

Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.

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Arijit K. De, Debjit Roy, and Debabrata Goswami "Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission," Journal of Biomedical Optics 16(10), 100505 (1 October 2011). https://doi.org/10.1117/1.3645082

Published: 1 October 2011

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