Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord

Yunzhou Shi; Riyi Shi; Ji-Xin Cheng; Delong Zhang; Terry B. Huff; Xiaofei Wang; Xiao-Ming Xu

doi:10.1117/1.3641988

1 October 2011 Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord

Yunzhou Shi, Riyi Shi, Ji-Xin Cheng, Delong Zhang, Terry B. Huff, Xiaofei Wang, Xiao-Ming Xu

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Journal of Biomedical Optics, Vol. 16, Issue 10, 106012 (October 2011). https://doi.org/10.1117/1.3641988

Abstract

In vivo imaging of white matter is important for the mechanistic understanding of demyelination and evaluation of remyelination therapies. Although white matter can be visualized by a strong coherent anti-Stokes Raman scattering (CARS) signal from axonal myelin, in vivo repetitive CARS imaging of the spinal cord remains a challenge due to complexities induced by the laminectomy surgery. We present a careful experimental design that enabled longitudinal CARS imaging of de- and remyelination at single axon level in live rats. In vivo CARS imaging of secretory phospholipase A₂ induced myelin vesiculation, macrophage uptake of myelin debris, and spontaneous remyelination by Schwann cells are sequentially monitored over a 3 week period. Longitudinal visualization of de- and remyelination at a single axon level provides a novel platform for rational design of therapies aimed at promoting myelin plasticity and repair.

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Yunzhou Shi, Riyi Shi, Ji-Xin Cheng, Delong Zhang, Terry B. Huff, Xiaofei Wang, and Xiao-Ming Xu "Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord," Journal of Biomedical Optics 16(10), 106012 (1 October 2011). https://doi.org/10.1117/1.3641988

Published: 1 October 2011

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