Biological reactivity of nanoparticles: mosaics from optical microscopy videos of giant lipid vesicles

Jernej Zupanc; Andrej Dobnikar; Damjana Drobne; Janez Valant; Deniz Erdogmus; Erhan Bas

doi:10.1117/1.3533319

1 February 2011 Biological reactivity of nanoparticles: mosaics from optical microscopy videos of giant lipid vesicles

Jernej Zupanc, Andrej Dobnikar, Damjana Drobne, Janez Valant, Deniz Erdogmus, Erhan Bas

Author Affiliations +

Funded by: NSF

Journal of Biomedical Optics, Vol. 16, Issue 2, 026003 (February 2011). https://doi.org/10.1117/1.3533319

Abstract

Emerging fields such as nanomedicine and nanotoxicology, demand new information on the effects of nanoparticles on biological membranes and lipid vesicles are suitable as an experimental model for bio-nano interaction studies. This paper describes image processing algorithms which stitch video sequences into mosaics and recording the shapes of thousands of lipid vesicles, which were used to assess the effect of CoFe₂O₄ nanoparticles on the population of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipid vesicles. The applicability of this methodology for assessing the potential of engineered nanoparticles to affect morphological properties of lipid membranes is discussed.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Jernej Zupanc, Andrej Dobnikar, Damjana Drobne, Janez Valant, Deniz Erdogmus, and Erhan Bas "Biological reactivity of nanoparticles: mosaics from optical microscopy videos of giant lipid vesicles," Journal of Biomedical Optics 16(2), 026003 (1 February 2011). https://doi.org/10.1117/1.3533319

Published: 1 February 2011

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