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1 August 2011 Raman spectroscopy detects deterioration in biomechanical properties of bone in a glucocorticoid-treated mouse model of rheumatoid arthritis
Jason R. Maher, Andrew J. Berger, Masahiko Takahata, Hani A. Awad
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Journal of Biomedical Optics, Vol. 16, Issue 8, 087012 (August 2011). https://doi.org/10.1117/1.3613933
Abstract
Although glucocorticoids are frequently prescribed for the symptomatic management of inflammatory disorders such as rheumatoid arthritis, extended glucocorticoid exposure is the leading cause of physician-induced osteoporosis and leaves patients at a high risk of fracture. To study the biochemical effects of glucocorticoid exposure and how they might affect biomechanical properties of the bone, Raman spectra were acquired from ex vivo tibiae of glucocorticoid- and placebo-treated wild-type mice and a transgenic mouse model of rheumatoid arthritis. Statistically significant spectral differences were observed due to both treatment regimen and mouse genotype. These differences are attributed to changes in the overall bone mineral composition, as well as the degree of phosphate mineralization in tibial cortical bone. In addition, partial least squares regression was used to generate a Raman-based prediction of each tibia's biomechanical strength as quantified by a torsion test. The Raman-based predictions were as accurate as those produced by microcomputed tomography derived parameters, and more accurate than the clinically-used parameter of bone mineral density. These results suggest that Raman spectroscopy could be a valuable tool for monitoring bone biochemistry in studies of bone diseases such as osteoporosis, including tests of drugs being developed to combat these diseases.
©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Jason R. Maher, Andrew J. Berger, Masahiko Takahata, and Hani A. Awad "Raman spectroscopy detects deterioration in biomechanical properties of bone in a glucocorticoid-treated mouse model of rheumatoid arthritis," Journal of Biomedical Optics 16(8), 087012 (1 August 2011). https://doi.org/10.1117/1.3613933
Published: 1 August 2011
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KEYWORDS
Raman spectroscopy
Bone
Minerals
Mouse models
Dual energy x-ray absorptiometry
Microcomputed tomography
Computed tomography
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