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25 July 2012 Classification of inflammatory bowel diseases by means of Raman spectroscopic imaging of epithelium cells
Christiane Bielecki, Claudio Marquardt, Andreas Stallmach, Thomas Bocklitz, Michael Schmitt, Jürgen Popp, Christoph Krafft, Akram Gharbi, Thomas Knosel
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Funded by: Thüringer Ministerium für Bildung, Wissenschaft und Kultur
Journal of Biomedical Optics, Vol. 17, Issue 7, 076030 (July 2012). https://doi.org/10.1117/1.JBO.17.7.076030
Abstract
We report on a Raman microspectroscopic characterization of the inflammatory bowel diseases (IBD) Crohn's disease (CD) and ulcerative colitis (UC). Therefore, Raman maps of human colon tissue sections were analyzed by utilizing innovative chemometric approaches. First, support vector machines were applied to highlight the tissue morphology ( = Raman spectroscopic histopathology). In a second step, the biochemical tissue composition has been studied by analyzing the epithelium Raman spectra of sections of healthy control subjects (n = 11), subjects with CD (n = 14), and subjects with UC (n = 13). These three groups exhibit significantly different molecular specific Raman signatures, allowing establishment of a classifier (support-vector-machine). By utilizing this classifier it was possible to separate between healthy control patients, patients with CD, and patients with UC with an accuracy of 98.90%. The automatic design of both classification steps (visualization of the tissue morphology and molecular classification of IBD) paves the way for an objective clinical diagnosis of IBD by means of Raman spectroscopy in combination with chemometric approaches.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Christiane Bielecki, Claudio Marquardt, Andreas Stallmach, Thomas Bocklitz, Michael Schmitt, Jürgen Popp, Christoph Krafft, Akram Gharbi, and Thomas Knosel "Classification of inflammatory bowel diseases by means of Raman spectroscopic imaging of epithelium cells," Journal of Biomedical Optics 17(7), 076030 (25 July 2012). https://doi.org/10.1117/1.JBO.17.7.076030
Published: 25 July 2012
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KEYWORDS
Raman spectroscopy
Tissues
Diagnostics
Spectroscopy
Imaging spectroscopy
Blood
Chemometrics
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