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9 July 2012 Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis
Bavishna B. Praveen, Praveen C. Ashok, Michael Mazilu, Kishan Dholakia, Andrew C. Riches, Simon Herrington
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Journal of Biomedical Optics, Vol. 17, Issue 7, 077006 (July 2012). https://doi.org/10.1117/1.JBO.17.7.077006
Abstract
In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (~ 30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Bavishna B. Praveen, Praveen C. Ashok, Michael Mazilu, Kishan Dholakia, Andrew C. Riches, and Simon Herrington "Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis," Journal of Biomedical Optics 17(7), 077006 (9 July 2012). https://doi.org/10.1117/1.JBO.17.7.077006
Published: 9 July 2012
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KEYWORDS
Raman spectroscopy
Luminescence
Tissues
Modulation
Signal to noise ratio
Bone
Tissue optics
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