Diffuse light reflectance signals as potential indicators of loss of viability in brain tissue due to hypoxia: charge-coupled-device-based imaging and fiber-based measurement

Satoko Kawauchi; Izumi Nishidate; Yoichi Uozumi; Hiroshi Nawashiro; Hiroshi Ashida; Shunichi Sato

doi:10.1117/1.JBO.18.1.015003

7 January 2013 Diffuse light reflectance signals as potential indicators of loss of viability in brain tissue due to hypoxia: charge-coupled-device-based imaging and fiber-based measurement

Satoko Kawauchi, Izumi Nishidate, Yoichi Uozumi, Hiroshi Nawashiro, Hiroshi Ashida, Shunichi Sato

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 1, 015003 (January 2013). https://doi.org/10.1117/1.JBO.18.1.015003

Abstract

Brain tissue is highly vulnerable to ischemia/hypoxia, and real-time monitoring of its viability is important. By fiber-based measurements for rat brain, we previously observed a unique triphasic reflectance change (TRC) after a certain period of time after hypoxia. After TRC, rats could not be rescued, suggesting that TRC can be used as an indicator of loss of brain tissue viability. In this study, we investigated this diffuse-reflectance change due to hypoxia in three parts. First, we developed and validated a theoretical method to quantify changes in the absorption and reduced scattering coefficients involved in TRC. Second, we performed charge-coupled-device-based reflectance imaging of the rat brain during hypoxia followed by reoxygenation to examine spatiotemporal characteristics of the reflectance and its correlation with reversibility of brain tissue damage. Third, we made simultaneous imaging and fiber-based measurement of the reflectance for the rat to compare signals obtained by these two modalities. We observed a nontriphasic reflectance change by the imaging, and it was associated with brain tissue viability. We found that TRC measured by the fibers preceded the reflectance-signal change captured by the imaging. This time difference is attributable to the different observation depths in the brain with these two methods.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Satoko Kawauchi, Izumi Nishidate, Yoichi Uozumi, Hiroshi Nawashiro, Hiroshi Ashida, and Shunichi Sato "Diffuse light reflectance signals as potential indicators of loss of viability in brain tissue due to hypoxia: charge-coupled-device-based imaging and fiber-based measurement," Journal of Biomedical Optics 18(1), 015003 (7 January 2013). https://doi.org/10.1117/1.JBO.18.1.015003

Published: 7 January 2013

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
14 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY