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3 October 2013 Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue
Mehmet S. Ozturk, Vivian K. Lee, Lingling Zhao, Guohoa Dai, Xavier Intes
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Funded by: American Heart Association Scientist Development, National Institutes of Health, National Science Foundation (NSF), NSF CAREER AWARD
Journal of Biomedical Optics, Vol. 18, Issue 10, 100501 (October 2013). https://doi.org/10.1117/1.JBO.18.10.100501
Abstract
Three-dimensional imaging of thick tissue constructs is one of the main challenges in the field of tissue engineering and regenerative medicine. Optical methods are the most promising as they offer noninvasive, fast, and inexpensive solutions. Herein, we report the use of mesoscopic fluorescence molecular tomography (MFMT) to image function and structure of thick bioprinted tissue hosted in a 3-mm-thick bioreactor. Collagen-based tissue assembled in this study contains two vascular channels formed by green fluorescent protein- and mCherry-expressing cells. Transfected live cell imaging enables us to image function, whereas Flash Red fluorescent bead perfusion into the vascular channel allows us to image structure. The MFMT optical reconstructions are benchmarked with classical microscopy techniques. MFMT and wide-field fluorescence microscopy data match within 92% in area and 84% in location, validating the accuracy of MFMT reconstructions. Our results demonstrate that MFMT is a well-suited imaging modality for fast, longitudinal, functional imaging of thick, and turbid tissue engineering constructs.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Mehmet S. Ozturk, Vivian K. Lee, Lingling Zhao, Guohoa Dai, and Xavier Intes "Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue," Journal of Biomedical Optics 18(10), 100501 (3 October 2013). https://doi.org/10.1117/1.JBO.18.10.100501
Published: 3 October 2013
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KEYWORDS
Tissues
Green fluorescent protein
Luminescence
Tomography
Microscopy
Fluorescence tomography
Tissue engineering
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