In vivo imaging of orthotopic prostate cancer with far-red gene reporter fluorescence tomography and in vivo and ex vivo validation

Yujie Lu; Chinmay D. Darne; I-Chih Tan; Grace Wu; Nathaniel Wilganowski; Holly Robinson; Ali Azhdarinia; Banghe Zhu; John C. Rasmussen; Eva Marie Sevick-Muraca

doi:10.1117/1.JBO.18.10.101305

25 June 2013 In vivo imaging of orthotopic prostate cancer with far-red gene reporter fluorescence tomography and in vivo and ex vivo validation

Yujie Lu, Chinmay D. Darne, I-Chih Tan, Grace Wu, Nathaniel Wilganowski, Holly Robinson, Ali Azhdarinia, Banghe Zhu, John C. Rasmussen, Eva Marie Sevick-Muraca

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Funded by: National Institute of Health Research, CPRIT

Journal of Biomedical Optics, Vol. 18, Issue 10, 101305 (June 2013). https://doi.org/10.1117/1.JBO.18.10.101305

Abstract

Fluorescence gene reporters have recently become available for excitation at far-red wavelengths, enabling opportunities for small animal in vivo gene reporter fluorescence tomography (GRFT). We employed multiple projections of the far-red fluorescence gene reporters IFP1.4 and iRFP, excited by a point source in transillumination geometry in order to reconstruct the location of orthotopically implanted human prostate cancer (PC3), which stably expresses the reporter. Reconstruction was performed using a linear radiative-transfer-based regularization-free tomographic method. Positron emission tomography (PET) imaging of a radiolabeled antibody-based agent that targeted epithelial cell adhesion molecule overexpressed on PC3 cells was used to confirm in vivo GRFT results. Validation of GRFT results was also conducted from ex vivo fluorescence imaging of resected prostate tumor. In addition, in mice with large primary prostate tumors, a combination of GRFT and PET showed that the radiolabeled antibody did not penetrate the tumor, consistent with known tumor transport limitations of large (∼150 kDa ) molecules. These results represent the first tomography of a living animal using far-red gene reporters.

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Yujie Lu, Chinmay D. Darne, I-Chih Tan, Grace Wu, Nathaniel Wilganowski, Holly Robinson, Ali Azhdarinia, Banghe Zhu, John C. Rasmussen, and Eva Marie Sevick-Muraca "In vivo imaging of orthotopic prostate cancer with far-red gene reporter fluorescence tomography and in vivo and ex vivo validation," Journal of Biomedical Optics 18(10), 101305 (25 June 2013). https://doi.org/10.1117/1.JBO.18.10.101305

Published: 25 June 2013

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