Deep-tissue photoacoustic tomography of Förster resonance energy transfer

Yu Wang; Jun Xia; Lihong V. Wang

doi:10.1117/1.JBO.18.10.101316

23 July 2013 Deep-tissue photoacoustic tomography of Förster resonance energy transfer

Yu Wang, Jun Xia, Lihong V. Wang

Author Affiliations +

Funded by: National Institutes of Health (NIH), National Institutes of Health, NIH, National Institute of Health, Network for Translational Research, National Institute of Health through, National Institutes of Health Grant, National Institutes of Health grants, National Institute of Health (NIH), NIH Director’s Pioneer Award, NIH Director’s Transformative Research Award, National Cancer Institute, National Academies Keck Futures Initiative grant IS 13, and National Institutes of Health, (NIH Director’s Pioneer Award), US National Institutes of Health (NIH), National Institute of Health or Wellcome Trust

Journal of Biomedical Optics, Vol. 18, Issue 10, 101316 (July 2013). https://doi.org/10.1117/1.JBO.18.10.101316

Abstract

Förster resonance energy transfer (FRET) is a distance-dependent process that transfers excited state energy from a donor molecule to an acceptor molecule without the emission of a photon. The FRET rate is determined by the proximity between the donor and the acceptor molecules; it becomes significant only when the proximity is within several nanometers. Therefore, FRET has been applied to visualize interactions and conformational changes of biomolecules, such as proteins, lipids, and nucleic acids that cannot be resolved by optical microscopy. Here, we report photoacoustic tomography of FRET efficiency at a 1-cm depth in chicken breast tissue, whereas conventional high-resolution fluorescence imaging is limited to <0.1 cm . Photoacoustic tomography is expected to facilitate the examination of FRET phenomena in living organisms.

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Yu Wang, Jun Xia, and Lihong V. Wang "Deep-tissue photoacoustic tomography of Förster resonance energy transfer," Journal of Biomedical Optics 18(10), 101316 (23 July 2013). https://doi.org/10.1117/1.JBO.18.10.101316

Published: 23 July 2013

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