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12 April 2013 In vivo visualization of dermal collagen fiber in skin burn by collagen-sensitive second-harmonic-generation microscopy
Ryosuke Tanaka, Shu-ichiro Fukushima, Kunihiko Sasaki, Yuji Tanaka, Hiroyuki Murota, Kenji Matsumoto, Tsutomu Araki, Takeshi Yasui
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Funded by: Grants-in-Aid for Scientific Research
Journal of Biomedical Optics, Vol. 18, Issue 6, 061231 (April 2013). https://doi.org/10.1117/1.JBO.18.6.061231
Abstract
Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy is demonstrated. Changes in signal vanishing patterns in the SHG images are confirmed to be dependent on the burn degree. Comparison of the SHG images with Masson’s trichrome-stained images indicated that the observed patterns were caused by the coexistence of molten and fibrous structures of dermal collagen fibers. Furthermore, a quantitative parameter for burn assessment based on the depth profile of the mean SHG intensity across the entire SHG image is proposed. These results and discussions imply a potential of SHG microscopy as a minimally invasive, highly quantitative tool for skin burn assessment.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Ryosuke Tanaka, Shu-ichiro Fukushima, Kunihiko Sasaki, Yuji Tanaka, Hiroyuki Murota, Kenji Matsumoto, Tsutomu Araki, and Takeshi Yasui "In vivo visualization of dermal collagen fiber in skin burn by collagen-sensitive second-harmonic-generation microscopy," Journal of Biomedical Optics 18(6), 061231 (12 April 2013). https://doi.org/10.1117/1.JBO.18.6.061231
Published: 12 April 2013
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Cited by 47 scholarly publications.
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KEYWORDS
Second-harmonic generation
Collagen
Skin
Microscopy
In vivo imaging
Visualization
Tissue optics
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