Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin

Te-Yu Tseng; Hsun-Chia Hsu; Chiu-Sheng Yang; Tsung-Hua Tsai; Chen-Yuan Dong

doi:10.1117/1.JBO.18.9.096016

25 September 2013 Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin

Te-Yu Tseng, Hsun-Chia Hsu, Chiu-Sheng Yang, Tsung-Hua Tsai, Chen-Yuan Dong

Author Affiliations +

Funded by: National Science Council, Taiwan, National Science Council, National Science Council in Taiwan, Ministry of Science and Technology, Taiwan (MOST), National Health Research Institute, National Health Research Institutes, National Taiwan University, Center for Quantum Science and Engineering of National Taiwan University

Journal of Biomedical Optics, Vol. 18, Issue 9, 096016 (September 2013). https://doi.org/10.1117/1.JBO.18.9.096016

Abstract

Transdermal drug delivery, transporting the drug molecules through epidermis to dermis, has been extensively investigated but not studied in dynamic detail. The objective of this study is to monitor the dynamical changes of drug permeation and that of polarization of skin tissue with oleic acid treatment. We utilize two-photon fluorescence microscopy (TPM) to investigate the dynamics of transdermal drug delivery in skin excised from the abdominal region of euthenized nude mice with sulforhodamine B (SRB) modeling as a drug and Laurdan serving as a polarity indicator. The treatment of oleic acid increases the permeation rate of SRB, quickly reaching to the steady state of permeation. Increases in polarity within the skin tissue (in both intercellular and intracellular region of stratum corneum) are observed in SRB permeation enhanced by oleic acid treatment. TPM has successfully demonstrated the ability to study spatial distribution of transport dynamics in oleic acid-enhanced transdermal delivery.

Citation Download Citation

Te-Yu Tseng, Hsun-Chia Hsu, Chiu-Sheng Yang, Tsung-Hua Tsai, and Chen-Yuan Dong "Investigation of transport dynamics in oleic acid–induced transdermal drug delivery by two-photon fluorescence microscopy: an ex-vivo study of mouse skin," Journal of Biomedical Optics 18(9), 096016 (25 September 2013). https://doi.org/10.1117/1.JBO.18.9.096016

Published: 25 September 2013

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 2 scholarly publications.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Molecules

Luminescence

Tissues

Two photon excitation microscopy

3D image processing

Photons

Show All Keywords

RELATED CONTENT

Measuring skin penetration under in-use conditions
Proceedings of SPIE (January 01 1900)

Fluorescence lifetime imaging of skin cancer
Proceedings of SPIE (February 17 2011)

Effects of index mismatch induced spherical aberration on two photon...
Proceedings of SPIE (July 10 2003)

Novel nanocarriers for topical drug delivery investigating delivery efficiency...
Proceedings of SPIE (February 22 2011)

Applications of two-photon fluorescence microscopy in deep-tissue imaging
Proceedings of SPIE (July 04 2000)

Fluorescence depolarization of normal and diseased skin tissues
Proceedings of SPIE (April 16 1998)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years