Integrated optical microfluidic biosensor using a polycarbazole photodetector for point-of-care detection of hormonal compounds

Nuno Pires; Tao Dong; Ulrik Hanke; Nils Hoivik

doi:10.1117/1.JBO.18.9.097001

3 September 2013 Integrated optical microfluidic biosensor using a polycarbazole photodetector for point-of-care detection of hormonal compounds

Nuno Pires, Tao Dong, Ulrik Hanke, Nils Hoivik

Author Affiliations +

Funded by: Norwegian Micro- and Nano-Fabrication Facility, NorFab

Journal of Biomedical Optics, Vol. 18, Issue 9, 097001 (September 2013). https://doi.org/10.1117/1.JBO.18.9.097001

Abstract

A picogram-sensitive optical microfluidic biosensor using an integrated polycarbazole photodiode is developed. The photodetector is mainly composed of the blend heterojunction of poly [N-9 ′ -heptadecanyl-2,7-carbazole-alt-5,5-(4 ′ ,7 ′ -di-2-thienyl-2 ′ ,1 ′ ,3 ′ -benzothiadiazole)] (PCDTBT) and [6,6]-phenyl C₇₁ -butyric acid methyl ester (PC ₇₀BM ) and the poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) as the hole transport layer. Analyte detection is accomplished via a chemiluminescent immunoassay performed in a poly(dimethylsiloxane)-gold-glass hybrid microchip, on which antibodies were immobilized and chemiluminescent horseradish peroxidase-luminol-peroxide reactions were generated. Enhanced sensor response to the chemiluminescent light is achieved by optimizing the thickness of PCDTBT: PC ₇₀ BM and PEDOT:PSS. Using the optimized polycarbazole photodiode for detecting the human thyroid-stimulating hormone as the model target, the integrated biosensor demonstrates an excellent linearity in the range of 0.03 to 10 ng/ml with an analytical sensitivity of 68 pg/ml . The sensor response shows high specificity and reproducibility. Hormone detection in clinical samples is further demonstrated and compared with a commercial enzyme-linked immunosorbent assay. The integrated device reported here has potential to detect other hormonal compounds or protein targets. Moreover, the presented concept enables the development of miniaturized, low-cost but highly sensitive optical microfluidic biosensors based on integrated polymer photodetectors with high potential for point-of-care diagnostics.

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Nuno Pires, Tao Dong, Ulrik Hanke, and Nils Hoivik "Integrated optical microfluidic biosensor using a polycarbazole photodetector for point-of-care detection of hormonal compounds," Journal of Biomedical Optics 18(9), 097001 (3 September 2013). https://doi.org/10.1117/1.JBO.18.9.097001

Published: 3 September 2013

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