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25 June 2013 High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography
Kyoohyun Kim, HyeOk Yoon, Monica Diez-Silva, Ming Dao, Ramachandra R. Dasari, YongKeun Park
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Journal of Biomedical Optics, Vol. 19, Issue 1, 011005 (June 2013). https://doi.org/10.1117/1.JBO.19.1.011005
Abstract
We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
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Kyoohyun Kim, HyeOk Yoon, Monica Diez-Silva, Ming Dao, Ramachandra R. Dasari, and YongKeun Park "High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography," Journal of Biomedical Optics 19(1), 011005 (25 June 2013). https://doi.org/10.1117/1.JBO.19.1.011005
Published: 25 June 2013
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Cited by 263 scholarly publications and 3 patents.
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KEYWORDS
Diffraction
Reconstruction algorithms
3D image processing
Tomography
Blood
Crystals
Optical tomography
CHORUS Article. This article was made freely available starting 25 June 2014
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