Lipid droplet pattern and nondroplet-like structure in two fat mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy

Yung-Hsiang Yi; Cheng-Hao Chien; Wei-Wen Chen; Tian-Hsiang Ma; Kuan-Yu Liu; Yu-Sun Chang; Ta-Chau Chang; Szecheng J. Lo

doi:10.1117/1.JBO.19.1.011011

26 August 2013 Lipid droplet pattern and nondroplet-like structure in two fat/i] mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy

Yung-Hsiang Yi, Cheng-Hao Chien, Wei-Wen Chen, Tian-Hsiang Ma, Kuan-Yu Liu, Yu-Sun Chang, Ta-Chau Chang, Szecheng J. Lo

Author Affiliations +
Yung-Hsiang Yi,¹ Cheng-Hao Chien,² Wei-Wen Chenhttps://orcid.org/0000-0003-2234-5686,² Tian-Hsiang Ma,¹ Kuan-Yu Liu,¹ Yu-Sun Chang,¹ Ta-Chau Chang,² Szecheng J. Lo¹

¹Chang Gung Univ. (Taiwan)
²Academia Sinica (Taiwan)

Journal of Biomedical Optics, Vol. 19, Issue 1, 011011 (August 2013). https://doi.org/10.1117/1.JBO.19.1.011011

ARTICLE

1 Introduction

2 Materials and Methods

2.1 Worm Strains and Reagents

2.2 Maintenance of C. elegans

2.3 Nile Red Staining and Colocalization Analysis

2.4 CARS/TPEF Microscopy and Lipid Droplet Quantification

3 Results and Discussion

3.1 Detecting Lipid Droplets in C. elegans by Nile Red Fluorescence and CARS Microscopy

3.2 Significant Decrease of Lipid Droplets in Fat-2 Mutant

3.3 NonDroplet-Like Structure with Enhanced CARS Signal in the Uterus of Fat-2 and Fat-3 Mutants

4 Conclusion

FIGURES & TABLES

REFERENCES

CITED BY

Google Scholar citations

Citing works

DOWNLOAD PAPER SAVE TO MY LIBRARY

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password
Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?
Show

Keep me signed in

No SPIE account? Create an account

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

Abstract

Lipid is an important energy source and essential component for plasma and organelle membranes in all kinds of cells. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free and nonlinear optical technique that can be used to monitor the lipid distribution in live organisms. Here, we utilize CARS microscopy to investigate the pattern of lipid droplets in two live Caenorhabditis elegans mutants (fat-2 and fat-3). The CARS images showed a striking decrease in the size, number, and content of lipid droplets in the fat-2 mutant but a slight difference in the fat-3 mutant as compared with the wild-type worm. Moreover, a nondroplet-like structure with enhanced CARS signal was detected for the first time in the uterus of fat-2 and fat-3 mutants. In addition, transgenic fat-2 mutant expressing a GFP fusion protein of vitellogenin-2 (a yolk lipoprotein) revealed that the enhanced CARS signal colocalized with the GFP signal, which suggests that the nondroplet-like structure is primarily due to the accumulation of yolk lipoproteins. Together, this study implies that CARS microscopy is a potential tool to study the distribution of yolk lipoproteins, in addition to lipid droplets, in live animals.

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Yung-Hsiang Yi, Cheng-Hao Chien, Wei-Wen Chen, Tian-Hsiang Ma, Kuan-Yu Liu, Yu-Sun Chang, Ta-Chau Chang, and Szecheng J. Lo "Lipid droplet pattern and nondroplet-like structure in two fat/i] mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy," Journal of Biomedical Optics 19(1), 011011 (26 August 2013). https://doi.org/10.1117/1.JBO.19.1.011011

Published: 26 August 2013

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS
Cited by 13 scholarly publications.

Explore citations on Lens.org

Advertisement

Advertisement

RIGHTS & PERMISSIONS
Get copyright permission

KEYWORDS

Microscopy

Uterus

Luminescence

Signal detection

CARS tomography

Green fluorescent protein

Mode conditioning cables

Organisms

Proteins

Visualization

Show All Keywords

RELATED CONTENT

The effect of polyunsaturated fatty acids on the homeostasis of...
Proceedings of SPIE (March 09 2016)

A new and highly sensitive method of analyzing metabolic activity...
Proceedings of SPIE (January 01 1900)

Non linear imaging techniques visualize the lipid profile of C....
Proceedings of SPIE (July 14 2015)

Unusual autofluorescence characteristic of cultured red-rain cells
Proceedings of SPIE (August 28 2008)

FRET microscopy to visualize transcription factor dimerization in the nucleus...
Proceedings of SPIE (April 24 2001)

In vivo monitoring specialized hepatocyte like cells in Drosophila by...
Proceedings of SPIE (February 09 2012)

Subscribe to Digital Library

Receive Erratum Email Alert

Erratum Email Alerts notify you when an article has been updated or the paper is withdrawn.

Visit My Account to manage your email alerts.

The alert successfully saved.

Visit My Account to manage your email alerts.

The alert did not successfully save. Please try again later.

Yung-Hsiang Yi, Cheng-Hao Chien, Wei-Wen Chen, Tian-Hsiang Ma, Kuan-Yu Liu, Yu-Sun Chang, Ta-Chau Chang, Szecheng J. Lo, "Lipid droplet pattern and nondroplet-like structure in two fat/i] mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy," J. Biomed. Opt. 19(1) 011011 (26 August 2013) https://doi.org/10.1117/1.JBO.19.1.011011

Include:

Citation Only

Citation & Abstract

Format:

RIS

EndNote

BibTex

Show All Keywords

Keywords/Phrases

Search In:

Publication Years