Dynamic fluorescence lifetime imaging based on acousto-optic deflectors

Wei Yan; Xiao Peng; Jing Qi; Jian Gao; Shunping Fan; Qi Wang; Junle Qu; Hanben Niu

doi:10.1117/1.JBO.19.11.116004

6 November 2014 Dynamic fluorescence lifetime imaging based on acousto-optic deflectors

Wei Yan, Xiao Peng, Jing Qi, Jian Gao, Shunping Fan, Qi Wang, Junle Qu, Hanben Niu

Author Affiliations +

Funded by: National Basic Research Program of China, National Natural Science Foundation of China, Shenzhen Science and Technology Development, China Postdoctoral Science Foundation

Journal of Biomedical Optics, Vol. 19, Issue 11, 116004 (November 2014). https://doi.org/10.1117/1.JBO.19.11.116004

Abstract

We report a dynamic fluorescence lifetime imaging (D-FLIM) system that is based on a pair of acousto-optic deflectors for the random regions of interest (ROI) study in the sample. The two-dimensional acousto-optic deflector devices are used to rapidly scan the femtosecond excitation laser beam across the sample, providing specific random access to the ROI. Our experimental results using standard fluorescent dyes in live cancer cells demonstrate that the D-FLIM system can dynamically monitor the changing process of the microenvironment in the ROI in live biological samples.

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Wei Yan, Xiao Peng, Jing Qi, Jian Gao, Shunping Fan, Qi Wang, Junle Qu, and Hanben Niu "Dynamic fluorescence lifetime imaging based on acousto-optic deflectors," Journal of Biomedical Optics 19(11), 116004 (6 November 2014). https://doi.org/10.1117/1.JBO.19.11.116004

Published: 6 November 2014

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