Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells

Yoochan Hong; Minhee Ku; Eugene Lee; Jin-Suck Suh; Yong-Min Huh; Dae-Sung Yoon; Jaemoon Yang

doi:10.1117/1.JBO.19.5.051202

2 December 2013 Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells

Yoochan Hong, Minhee Ku, Eugene Lee, Jin-Suck Suh, Yong-Min Huh, Dae-Sung Yoon, Jaemoon Yang

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Journal of Biomedical Optics, Vol. 19, Issue 5, 051202 (December 2013). https://doi.org/10.1117/1.JBO.19.5.051202

Abstract

In this study, we describe the development of a cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance that enables recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of the sensing chip was confirmed using various solvents that have different refractive indexes. Subsequently, MT1-MMP–specific cleavable peptide was conjugated onto the surface of GNRs, and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed a biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.

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Yoochan Hong, Minhee Ku, Eugene Lee, Jin-Suck Suh, Yong-Min Huh, Dae-Sung Yoon, and Jaemoon Yang "Localized surface plasmon resonance based nanobiosensor for biomarker detection of invasive cancer cells," Journal of Biomedical Optics 19(5), 051202 (2 December 2013). https://doi.org/10.1117/1.JBO.19.5.051202

Published: 2 December 2013

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