Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix

Vidita Urboniene; Milda Pucetaite; Feliksas Jankevicius; Arunas Zelvys; Valdas Sablinskas; Gerald Steiner

doi:10.1117/1.JBO.19.8.087005

20 August 2014 Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix

Vidita Urboniene, Milda Pucetaite, Feliksas Jankevicius, Arunas Zelvys, Valdas Sablinskas, Gerald Steiner

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Journal of Biomedical Optics, Vol. 19, Issue 8, 087005 (August 2014). https://doi.org/10.1117/1.JBO.19.8.087005

Abstract

Fourier transform infrared (FT-IR) spectroscopy was applied to characterize the extracellular matrix (ECM) of kidney tumor tissue and normal kidney tissue. Freshly resected tissue samples from 31 patients were pressed on a CaF ₂ substrate. FT-IR spectra obtained from ECM of tumor tissue exhibit stronger absorption bands in the spectral region from 1000 to 1200 cm ⁻¹ and around 1750 cm ⁻¹ than those obtained from normal tissue. It is likely that the spectra of ECM of kidney tumor tissue with large increases in the intensities of these bands represent a higher concentration of fatty acids and glycerol. Amide I and amide II bands are stronger in the spectra of ECM from normal tissue, indicating a higher level of proteins. Our results suggest that FT-IR spectroscopy of the ECM is an innovative emerging technology for real-time intraoperative tumor diagnosis, which may improve margin clearance in renal cancer surgery.

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Vidita Urboniene, Milda Pucetaite, Feliksas Jankevicius, Arunas Zelvys, Valdas Sablinskas, and Gerald Steiner "Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix," Journal of Biomedical Optics 19(8), 087005 (20 August 2014). https://doi.org/10.1117/1.JBO.19.8.087005

Published: 20 August 2014

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