Induction and imaging of photothrombotic stroke in conscious and freely moving rats

Hongyang Lu; Yao Li; Lu Yuan; Hangdao Li; Xiaodan Lu; Shanbao Tong

doi:10.1117/1.JBO.19.9.096013

19 September 2014 Induction and imaging of photothrombotic stroke in conscious and freely moving rats

Hongyang Lu, Yao Li, Lu Yuan, Hangdao Li, Xiaodan Lu, Shanbao Tong

Author Affiliations +

Funded by: National Natural Science Foundation of China, NSFC, National Science Foundation of China

Journal of Biomedical Optics, Vol. 19, Issue 9, 096013 (September 2014). https://doi.org/10.1117/1.JBO.19.9.096013

Abstract

In experimental stroke research, anesthesia is common and serves as a major reason for translational failure. Real-time cerebral blood flow (CBF) monitoring during stroke onset can provide important information for the prediction of brain injury; however, this is difficult to achieve in clinical practice due to various technical problems. We created a photothrombotic focal ischemic stroke model utilizing our self-developed miniature headstage in conscious and freely moving rats. In this model, a high spatiotemporal resolution imager using laser speckle contrast imaging technology was integrated to acquire real-time two-dimensional CBF information during thrombosis. The feasibility, stability, and reliability of the system were tested in terms of CBF, behavior, and T2-weighted magnetic resonance imaging (MRI) findings. After completion of occlusion, the CBF in the targeted cortex of the stroke group was reduced to 16±9% of the baseline value. The mean infarct volume measured by MRI 24 h postmodeling was 77±11 mm³ and correlated well with CBF (R²=0.74). This rodent model of focal cerebral ischemia and real-time blood flow imaging opens the possibility of performing various fundamental and translational studies on stroke without the influence of anesthetics.

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Hongyang Lu, Yao Li, Lu Yuan, Hangdao Li, Xiaodan Lu, and Shanbao Tong "Induction and imaging of photothrombotic stroke in conscious and freely moving rats," Journal of Biomedical Optics 19(9), 096013 (19 September 2014). https://doi.org/10.1117/1.JBO.19.9.096013

Published: 19 September 2014

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