Intraoperative laser speckle contrast imaging improves the stability of rodent middle cerebral artery occlusion model

Lu Yuan; Yao Li; Hangdao Li; Hongyang Lu; Shanbao Tong

doi:10.1117/1.JBO.20.9.096012

11 September 2015 Intraoperative laser speckle contrast imaging improves the stability of rodent middle cerebral artery occlusion model

Lu Yuan, Yao Li, Hangdao Li, Hongyang Lu, Shanbao Tong

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Funded by: National Natural Science Foundation of China, China Scholarship Council

Journal of Biomedical Optics, Vol. 20, Issue 9, 096012 (September 2015). https://doi.org/10.1117/1.JBO.20.9.096012

Abstract

Rodent middle cerebral artery occlusion (MCAO) model is commonly used in stroke research. Creating a stable infarct volume has always been challenging for technicians due to the variances of animal anatomy and surgical operations. The depth of filament suture advancement strongly influences the infarct volume as well. We investigated the cerebral blood flow (CBF) changes in the affected cortex using laser speckle contrast imaging when advancing suture during MCAO surgery. The relative CBF drop area (CBF₅₀, i.e., the percentage area with CBF less than 50% of the baseline) showed an increase from 20.9% to 69.1% when the insertion depth increased from 1.6 to 1.8 cm. Using the real-time CBF₅₀ marker to guide suture insertion during the surgery, our animal experiments showed that intraoperative CBF-guided surgery could significantly improve the stability of MCAO with a more consistent infarct volume and less mortality.

Yuan, Li, Li, Lu, and Tong: Intraoperative laser speckle contrast imaging improves the stability of rodent middle cerebral artery occlusion model

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Lu Yuan, Yao Li, Hangdao Li, Hongyang Lu, and Shanbao Tong "Intraoperative laser speckle contrast imaging improves the stability of rodent middle cerebral artery occlusion model," Journal of Biomedical Optics 20(9), 096012 (11 September 2015). https://doi.org/10.1117/1.JBO.20.9.096012

Published: 11 September 2015

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