Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion

Mike Friedrich; Gregory S. Harms

doi:10.1117/1.JBO.20.10.106006

15 October 2015 Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion

Mike Friedrich, Gregory S. Harms

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Funded by: German Research Foundation (DFG)

Journal of Biomedical Optics, Vol. 20, Issue 10, 106006 (October 2015). https://doi.org/10.1117/1.JBO.20.10.106006

Abstract

Planar illumination imaging allows for illumination of the focal plane orthogonal to the imaging axis in various light forms and is advantageous for high optical sectioning, high imaging speed, low light exposure, and inherently deeper imaging penetration into small organisms and tissue sections. The drawback of the technique is the low inherent resolution, which can be overcome by the incorporation of a dual-sheet stimulated emission depletion (STED) beam to the planar illumination excitation. Our initiative is the implementation of STED into the planar illumination microscope for enhanced resolution. We demonstrate some of our implementations. The depletion of STED in the microscope follows an inverse square root saturation for up to 2.5-fold axial resolution improvements with both high and low numerical aperture imaging objectives.

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Mike Friedrich and Gregory S. Harms "Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion," Journal of Biomedical Optics 20(10), 106006 (15 October 2015). https://doi.org/10.1117/1.JBO.20.10.106006

Published: 15 October 2015

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