CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 21 > Issue 10 > Article
Open Access
20 October 2016 Study of the inhibition effect of thiazone on muscle optical clearing
Xiaowei Jin, Zhichao Deng, Jin Wang, Qing Ye, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Jianguo Tian
Author Affiliations +
Journal of Biomedical Optics, Vol. 21, Issue 10, 105004 (October 2016). https://doi.org/10.1117/1.JBO.21.10.105004
Abstract
We investigated the effect of thiazone, a widely used penetration enhancer, on in vitro porcine skin and muscle tissue by single-integrating sphere technique during optical clearing (OC) treatment. The results showed that thiazone induced an increase on the total transmittance of skin which led to a reduction in that of muscle in the spectral range from 400 to 800 nm. Small particles crystalized out from the thiazone-treated muscle were observed by microscopy imaging. With the help of x-ray diffraction measurement, we ascertained that the crystal was a single-crystal of thiazone, which mainly induced an increase of the scattering. Contrast transmittance measurements carried on the mixture of water, thizaone–propylene glycol solution showed that the free water in muscle could be the main reason for the thiazone crystallization. Therefore, during OC treatment of thiazone, the remarkable effect on skin and the noticeable inhibition effect on subcutaneous muscle tissue after penetrating into the skin should be considered. The experimental results provide such a reference for the choice of penetration enhancer.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2016/$25.00 © 2016 SPIE
Citation Download Citation
Xiaowei Jin, Zhichao Deng, Jin Wang, Qing Ye, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, and Jianguo Tian "Study of the inhibition effect of thiazone on muscle optical clearing," Journal of Biomedical Optics 21(10), 105004 (20 October 2016). https://doi.org/10.1117/1.JBO.21.10.105004
Published: 20 October 2016
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 5 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Skin
Transmittance
Crystals
In vitro testing
Optical clearing
Tissues
Scattering
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top