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27 October 2016 Spatially multiplexed interferometric microscopy with partially coherent illumination
José Ángel Picazo-Bueno, Zeev Zalevsky, Javier García, Carlos Ferreira, Vicente Micó
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Journal of Biomedical Optics, Vol. 21, Issue 10, 106007 (October 2016). https://doi.org/10.1117/1.JBO.21.10.106007
Abstract
We have recently reported on a simple, low cost, and highly stable way to convert a standard microscope into a holographic one [Opt. Express 22, 14929 (2014)]. The method, named spatially multiplexed interferometric microscopy (SMIM), proposes an off-axis holographic architecture implemented onto a regular (nonholographic) microscope with minimum modifications: the use of coherent illumination and a properly placed and selected one-dimensional diffraction grating. In this contribution, we report on the implementation of partially (temporally reduced) coherent illumination in SMIM as a way to improve quantitative phase imaging. The use of low coherence sources forces the application of phase shifting algorithm instead of off-axis holographic recording to recover the sample’s phase information but improves phase reconstruction due to coherence noise reduction. In addition, a less restrictive field of view limitation (1/2) is implemented in comparison with our previously reported scheme (1/3). The proposed modification is experimentally validated in a regular Olympus BX-60 upright microscope considering a wide range of samples (resolution test, microbeads, swine sperm cells, red blood cells, and prostate cancer cells).
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2016/$25.00 © 2016 SPIE
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José Ángel Picazo-Bueno, Zeev Zalevsky, Javier García, Carlos Ferreira, and Vicente Micó "Spatially multiplexed interferometric microscopy with partially coherent illumination," Journal of Biomedical Optics 21(10), 106007 (27 October 2016). https://doi.org/10.1117/1.JBO.21.10.106007
Published: 27 October 2016
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KEYWORDS
Microscopes
Multiplexing
Interferometry
Holography
Microscopy
Diffraction gratings
Phase shifts
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