Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue

Hongfu Zhou; Yadong Gang; Shenghua Chen; Yu Wang; Yumiao Xiong; Longhui Li; Fangfang Yin; Yue Liu; Xiuli Liu; Shaoqun Zeng

doi:10.1117/1.JBO.22.10.106015

27 October 2017 Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue

Hongfu Zhou, Yadong Gang, Shenghua Chen, Yu Wang, Yumiao Xiong, Longhui Li, Fangfang Yin, Yue Liu, Xiuli Liu, Shaoqun Zeng

Author Affiliations +

Funded by: National Basic Research Program of China, Program 973, Science Fund for Creative Research Group of China, Science Fund for Creative Research Group, NSFC projects

Journal of Biomedical Optics, Vol. 22, Issue 10, 106015 (October 2017). https://doi.org/10.1117/1.JBO.22.10.106015

Abstract

Plastic embedding is widely applied in light microscopy analyses. Previous studies have shown that embedding agents and related techniques can greatly affect the quality of biological tissue embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial embedding resins and related embedding techniques directly. Here, we developed a neutral embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.

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Hongfu Zhou, Yadong Gang, Shenghua Chen, Yu Wang, Yumiao Xiong, Longhui Li, Fangfang Yin, Yue Liu, Xiuli Liu, and Shaoqun Zeng "Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue," Journal of Biomedical Optics 22(10), 106015 (27 October 2017). https://doi.org/10.1117/1.JBO.22.10.106015

Received: 5 July 2017; Accepted: 2 October 2017; Published: 27 October 2017

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