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16 February 2018 Fiber bundle endomicroscopy with multi-illumination for three-dimensional reflectance image reconstruction
Yoriko Ando, Hirohito Sawahata, Takeshi Kawano, Kowa Koida, Rika Numano
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Funded by: Research Foundation for Opto-Science and Technology, MEXT/the Promotion of Science (JSPS) KAKENHI, Takeda Science Foundation, Toyohashi University of Technology
Journal of Biomedical Optics, Vol. 23, Issue 2, 020502 (February 2018). https://doi.org/10.1117/1.JBO.23.2.020502
Abstract
Bundled fiber optics allow in vivo imaging at deep sites in a body. The intrinsic optical contrast detects detailed structures in blood vessels and organs. We developed a bundled-fiber-coupled endomicroscope, enabling stereoscopic three-dimensional (3-D) reflectance imaging with a multipositional illumination scheme. Two illumination sites were attached to obtain reflectance images with left and right illumination. Depth was estimated by the horizontal disparity between the two images under alternative illuminations and was calibrated by the targets with known depths. This depth reconstruction was applied to an animal model to obtain the 3-D structure of blood vessels of the cerebral cortex (Cereb cortex) and preputial gland (Pre gla). The 3-D endomicroscope could be instrumental to microlevel reflectance imaging, improving the precision in subjective depth perception, spatial orientation, and identification of anatomical structures.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Yoriko Ando, Hirohito Sawahata, Takeshi Kawano, Kowa Koida, and Rika Numano "Fiber bundle endomicroscopy with multi-illumination for three-dimensional reflectance image reconstruction," Journal of Biomedical Optics 23(2), 020502 (16 February 2018). https://doi.org/10.1117/1.JBO.23.2.020502
Received: 24 September 2017; Accepted: 17 January 2018; Published: 16 February 2018
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KEYWORDS
Reflectivity
3D acquisition
3D image processing
Blood vessels
Positron emission tomography
Endomicroscopy
Image restoration
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