CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 24 > Issue 3 > Article
Open Access
4 September 2018 Aberration correction method based on double-helix point spread function
Zhaojun Wang, Yanan Cai, Yansheng Liang, Dan Dan, Baoli Yao, Ming Lei
Author Affiliations +
Journal of Biomedical Optics, Vol. 24, Issue 3, 031005 (September 2018). https://doi.org/10.1117/1.JBO.24.3.031005
Abstract
Point spread function (PSF) engineering has met with lots of interest in various optical imaging techniques, including super-resolution microscopy, microparticle tracking, and extended depth-of-field microscopy. The intensity distributions of the modified PSFs often suffer from deteriorations caused by system aberrations, which greatly degrade the image contrast, resolution, or localization precision. We present an aberration correction method using a spiral-phase-based double-helix PSF as an aberration indicator, which is sensitive and quantitatively correlated to the spherical aberration, coma, and astigmatism. Superior to the routine iteration-based correction methods, the presented approach is iteration-free and the aberration coefficients can be directly calculated with the measured parameters, relieving the computing burden. The validity of the method is verified by both examining the intensity distribution of the conventional Gaussian PSF in three dimensions and observing muntjac skin fibroblast cells. This iteration-free correction method has a potential application in PSF engineering systems equipped with a spatial light modulator.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Zhaojun Wang, Yanan Cai, Yansheng Liang, Dan Dan, Baoli Yao, and Ming Lei "Aberration correction method based on double-helix point spread function," Journal of Biomedical Optics 24(3), 031005 (4 September 2018). https://doi.org/10.1117/1.JBO.24.3.031005
Received: 23 June 2018; Accepted: 16 August 2018; Published: 4 September 2018
JOURNAL ARTICLE
 11 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 7 scholarly publications.
Advertisement
Advertisement
KEYWORDS
Monochromatic aberrations
Point spread functions
Aberration correction
Spatial light modulators
Wavefront sensors
Computer generated holography
Imaging systems
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top