Measurement of the refractive index of whole blood and its components for a continuous spectral region

Shike Liu; Zhichao Deng; Jianwei Li; Jin Wang; Ningning Huang; Ruiming Cui; Qiannan Zhang; Jianchun Mei; Wenyuan Zhou; Chunping Zhang; Qing Ye; Jianguo Tian

doi:10.1117/1.JBO.24.3.035003

7 March 2019 Measurement of the refractive index of whole blood and its components for a continuous spectral region

Shike Liu, Zhichao Deng, Jianwei Li, Jin Wang, Ningning Huang, Ruiming Cui, Qiannan Zhang, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Qing Ye, Jianguo Tian

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Journal of Biomedical Optics, Vol. 24, Issue 3, 035003 (March 2019). https://doi.org/10.1117/1.JBO.24.3.035003

Abstract

The refractive index of blood is a key biophysical parameter, which can reflect the physiological state. We measured the refractive index of whole blood and other components, such as serum, plasma, and hemoglobin, based on internal reflection by using a homemade apparatus in the spectral range of 400 to 750 nm. In addition to the hemoglobin solution, which has a Soret band about 420 nm and two Q-bands between 500 and 600 nm, the measurements of other samples are the normal dispersion curve. The results are approximated by the Cauchy equation and Sellmeier equation, and the correlation coefficients are more than 0.997.

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Shike Liu, Zhichao Deng, Jianwei Li, Jin Wang, Ningning Huang, Ruiming Cui, Qiannan Zhang, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Qing Ye, and Jianguo Tian "Measurement of the refractive index of whole blood and its components for a continuous spectral region," Journal of Biomedical Optics 24(3), 035003 (7 March 2019). https://doi.org/10.1117/1.JBO.24.3.035003

Received: 16 November 2018; Accepted: 12 February 2019; Published: 7 March 2019

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