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20 December 2024 To label or not: the need for validation in label-free imaging
Joseph M. Szulczewski, Filiz Yesilkoy, Tyler Ulland, Randy Bartels, Bryan A. Millis, Stephen A. Boppart, Kevin W. Eliceiri
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Journal of Biomedical Optics, Vol. 29, Issue S2, S22717 (December 2024). https://doi.org/10.1117/1.JBO.29.S2.S22717
Abstract

Significance

Advances in label-free imaging have impacted many areas of biological and biomedical imaging ranging from cell biology and cancer to pathology and neuroscience. Despite the great progress and advantages of these methods, it is clear that to realize their full potential, validation by extrinsic labels and probes is critically needed.

Aim

This perspective calls for developing and applying innovative labels and probes to validate both existing and emerging label-free imaging methods.

Approach

Major representative types of label-free imaging methods are briefly presented discussing their advantages and differing contrasts. Their biological applications are also reviewed with a focus on how validation of label-free methods with carefully developed labeling approaches will greatly aid in further intrinsic contrast imaging adoption and likely lead to more sophisticated image-based biomarkers and a better understanding of the underlying signals.

Conclusions

Expanded efforts in extrinsic label validation will significantly push forward the utilization and adoption of label-free methods both in basic research and clinical models.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Citation Download Citation
Joseph M. Szulczewski, Filiz Yesilkoy, Tyler Ulland, Randy Bartels, Bryan A. Millis, Stephen A. Boppart, and Kevin W. Eliceiri "To label or not: the need for validation in label-free imaging," Journal of Biomedical Optics 29(S2), S22717 (20 December 2024). https://doi.org/10.1117/1.JBO.29.S2.S22717
Received: 4 October 2024; Accepted: 10 December 2024; Published: 20 December 2024
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KEYWORDS
Biological imaging
Biomedical optics
Imaging spectroscopy
Raman spectroscopy
Spectroscopy
Vibration
Autofluorescence
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