Open Access
1 September 2004 Two-photon laser scanning fluorescence microscopy using photonic crystal fiber
Gail McConnell, Erling Riis
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Abstract
We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiaton of less than 35 fs duration reaching the sample.
©(2004) Society of Photo-Optical Instrumentation Engineers (SPIE)
Gail McConnell and Erling Riis "Two-photon laser scanning fluorescence microscopy using photonic crystal fiber," Journal of Biomedical Optics 9(5), (1 September 2004). https://doi.org/10.1117/1.1778734
Published: 1 September 2004
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CITATIONS
Cited by 49 scholarly publications and 3 patents.
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KEYWORDS
Luminescence

Dispersion

Sapphire lasers

Microscopes

Microscopy

Laser scanners

Head

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