Plasmon-enhanced fluorescence of labeled biomolecules on top of a silver sol-gel film

Svetlana V. Vaschenko; Andrei Ramanenka; Dmitry V. Guzatov; Vyacheslav V. Stankevich; Anatoly Ya. Lunevich; Yuri F. Glukhov; Iosiph F. Sveklo; Sergey V. Gaponenko

doi:10.1117/1.JNP.6.061710

16 October 2012 Plasmon-enhanced fluorescence of labeled biomolecules on top of a silver sol-gel film

Svetlana V. Vaschenko, Andrei Ramanenka, Dmitry V. Guzatov, Vyacheslav V. Stankevich, Anatoly Ya. Lunevich, Yuri F. Glukhov, Iosiph F. Sveklo, Sergey V. Gaponenko

Author Affiliations +

Journal of Nanophotonics, Vol. 6, Issue 1, 061710 (October 2012). https://doi.org/10.1117/1.JNP.6.061710

Abstract

A simple chemical technique was devised for the fabrication of silver nanostructured substrates which can be used for plasmonic enhancement of labeled proteins fluorescence. For bovine serum albumin labeled with fluorescein isothiocyanate, the obtained enhancement factor ranges from three to seven, depending on metal-luminophore spacing and silver nanoparticle size. For excitation with linear polarized light, the enhancement factor increases noticeably for p -polarization and decreases for s -polarization. The experimental results were interpreted in terms of the theoretical model in which the enhancement factor depends on incident light polarization, luminophoremetal spacing and silver nanoparticle size. Proposed plasmonic substrates can be considered as an affordable replacement of standard ones in different types of fluorescent assays for the purpose of increasing sensitivity.

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Svetlana V. Vaschenko, Andrei Ramanenka, Dmitry V. Guzatov, Vyacheslav V. Stankevich, Anatoly Ya. Lunevich, Yuri F. Glukhov, Iosiph F. Sveklo, and Sergey V. Gaponenko "Plasmon-enhanced fluorescence of labeled biomolecules on top of a silver sol-gel film," Journal of Nanophotonics 6(1), 061710 (16 October 2012). https://doi.org/10.1117/1.JNP.6.061710

Published: 16 October 2012

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