CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > Neurophoton. > Volume 4 > Issue 1 > Article
Open Access
9 February 2017 Imaging extracellular potassium dynamics in brain tissue using a potassium-sensitive nanosensor
Joel Wellbourne-Wood, Theresa S. Rimmele, Jean-Yves Chatton
Author Affiliations +
Funded by: Swiss National Science Foundation
Neurophotonics, Vol. 4, Issue 1, 015002 (February 2017). https://doi.org/10.1117/1.NPh.4.1.015002
Abstract
Neuronal activity results in the release of K+ into the extracellular space (ECS). Classically, measurements of extracellular K+ ([K+]o) are carried out using K+-sensitive microelectrodes, which provide a single point measurement with undefined spatial resolution. An imaging approach would enable the spatiotemporal mapping of [K+]o. Here, we report on the design and characterization of a fluorescence imaging-based K+-sensitive nanosensor for the ECS based on dendrimer nanotechnology. Spectral characterization, sensitivity, and selectivity of the nanosensor were assessed by spectrofluorimetry, as well as in both wide-field and two-photon microscopy settings, demonstrating the nanosensor efficacy over the physiologically relevant ion concentration range. Spatial and temporal kinetics of the nanosensor responses were assessed using a localized iontophoretic K+ application on a two-photon imaging setup. Using acute mouse brain slices, we demonstrate that the nanosensor is retained in the ECS for extended periods of time. In addition, we present a ratiometric ve
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Joel Wellbourne-Wood, Theresa S. Rimmele, and Jean-Yves Chatton "Imaging extracellular potassium dynamics in brain tissue using a potassium-sensitive nanosensor," Neurophotonics 4(1), 015002 (9 February 2017). https://doi.org/10.1117/1.NPh.4.1.015002
Received: 9 December 2016; Accepted: 19 January 2017; Published: 9 February 2017
JOURNAL ARTICLE
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 16 scholarly publications and 1 patent.
Advertisement
Advertisement
KEYWORDS
Potassium
Nanosensors
Luminescence
Brain
Dendrimers
Tissues
Neuroimaging
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top