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5 April 2017 Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation
Gleb P. Tolstykh, Cory A. Olsovsky, Bennett L. Ibey, Hope T. Beier
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Neurophotonics, Vol. 4, Issue 2, 025001 (April 2017). https://doi.org/10.1117/1.NPh.4.2.025001
Abstract
Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from IR exposure could activate temperature- or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. Studies using fluorescent intensity-based calcium ion ( Ca 2 + ) sensitive dyes show changes in Ca 2 + levels after various IR stimulation parameters, which suggests that Ca 2 + may originate from the external solution. However, activation of intracellular signaling pathways has also been demonstrated, indicating a more complex mechanism of increasing intracellular Ca 2 + concentration. We quantified the Ca 2 + mobilization in terms of influx from the external solution and efflux from intracellular organelles using Fura-2 and a high-speed ratiometric imaging system that rapidly alternates the dye excitation wavelengths. Using nonexcitable Chinese hamster ovarian ( CHO - hM 1 ) cells and neuroblastoma-glioma (NG108) cells, we demonstrate that intracellular IP 3 receptors play an important role in the IR-induced Ca 2 + , with the © 2017 Society of Photo-Optical Instrumentation Engineers (SPIE) 2329-423X/2017/$25.00 © 2017 SPIE
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Gleb P. Tolstykh, Cory A. Olsovsky, Bennett L. Ibey, and Hope T. Beier "Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation," Neurophotonics 4(2), 025001 (5 April 2017). https://doi.org/10.1117/1.NPh.4.2.025001
Received: 1 December 2016; Accepted: 20 March 2017; Published: 5 April 2017
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Cited by 13 scholarly publications.
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KEYWORDS
Calcium
Receptors
Plasma
Infrared lasers
Modulation
Infrared radiation
Infrared imaging
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