Reconstruction Of 3D Light Microscopic Images

Gerhard Zinser; Angelika Erhardt; Josef Bille

doi:10.1117/12.944882

4 December 1984 Reconstruction Of 3D Light Microscopic Images

Gerhard Zinser, Angelika Erhardt, Josef Bille

Proceedings Volume 0504, Applications of Digital Image Processing VII; (1984) https://doi.org/10.1117/12.944882
Event: 28th Annual Technical Symposium, 1984, San Diego, United States

Abstract

Light-microscopic images of three-dimensional objects are distorted by defocused projections into the focus plane. A reconstruction procedure for three-dimensional light-microscopic images based on the linear system theory is described. Reconstruction is executed by inverse filtering of the image using an approximation of the three-dimen-sional optical transfer function of the microscope. The derivation and the features of this transfer function are out-lined. The reconstruction procedure permits to achieve a significant improvement of the image quality. The recon-struction result of a test object is shown.

Citation Download Citation

Gerhard Zinser, Angelika Erhardt, and Josef Bille "Reconstruction Of 3D Light Microscopic Images", Proc. SPIE 0504, Applications of Digital Image Processing VII, (4 December 1984); https://doi.org/10.1117/12.944882

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