Phosphorescence lifetime measurement using confocal microscopy

Chengjun Li; Shaoqun Zeng; Qingming Luo; Wei Zhou; Guoqing Zhang

doi:10.1117/12.462566

12 April 2002 Phosphorescence lifetime measurement using confocal microscopy

Chengjun Li, Shaoqun Zeng, Qingming Luo, Wei Zhou, Guoqing Zhang

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462566
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

The continuous light coming from the Argon Gas Ion Laser can be converted to pulse light when an optical chopper is equipped. The sample is excited by the pulse light in the confocal microscopy system. The light intensity of the excitation light and the phosphorescence is simultaneously recorded in terms of gray value by the confocal microscopy system. The phosphorescence lifetime measurement can be realized with time-resolved analysis for the phosphorescence intensity decay. The different decay lifetimes of the Oxy- Phor R2 sample under different oxygen concentration are measured with the reconstructed confocal microscopy system.

Citation Download Citation

Chengjun Li, Shaoqun Zeng, Qingming Luo, Wei Zhou, and Guoqing Zhang "Phosphorescence lifetime measurement using confocal microscopy", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462566

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