Dr. Anja Mittag Profile

Dr. Anja Mittag

at Univ Leipzig

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Author

Publications (18)

Proceedings Article | 4 March 2014 Paper

Diagnosis of myocardial infarction based on lectin-induced erythrocyte agglutination: a feasibility study

József Bocsi, Kathleen Nieschke, Anja Mittag, Thomas Reichert, Wiebke Laffers, Monika Marecka, Arkadiusz Pierzchalski, Joachim Piltz, Hans-Jürgen Esche, Günther Wolf, Ingo Dähnert, Adolf Baumgartner, Attila Tarnok

Proceedings Volume 8947, 89470V (2014) https://doi.org/10.1117/12.2037702

KEYWORDS: Blood, Absorbance, Diagnostics, Particles, Chest, Light scattering, Heart, Mathematical modeling, Electrocardiography, Proteins

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Myocardial infarction (MI) is an acute life-threatening disease with a high incidence worldwide. Aim of this study was to test lectin-carbohydrate binding-induced red blood cell (RBC) agglutination as an innovative tool for fast, precise and cost effective diagnosis of MI. Five lectins (Ricinus communis agglutinin (RCA), Phaseolus vulgaris erythroagglutinin (PHA), Datura stramonium agglutinin (DSA), Artocarpus agglutinin (ArA), Triticum agglutinin (TA)) were tested for ability to differentiate between agglutination characteristics in patients with MI (n = 101) or angina pectoris without MI (AP) (n = 34) and healthy volunteers (HV) as control (n =68) . RBC agglutination was analyzed by light absorbance of a stirred RBC suspension in the green to red light spectrum in an agglutimeter (amtec, Leipzig, Germany) for 15 min after lectin addition. Mean cell count in aggregates was estimated from light absorbance by a mathematical model. Each lectin induced RBC agglutination. RCA led to the strongest RBC agglutination (~500 RBCs/aggregate), while the others induced substantially slower agglutination and lead to smaller aggregate sizes (5-150 RBCs/aggregate). For all analyzed lectins the lectin-induced RBC agglutination of MI or AP patients was generally higher than for HV. However, only PHA induced agglutination that clearly distinguished MI from HV. Variance analysis showed that aggregate size after 15 min. agglutination induced by PHA was significantly higher in the MI group (143 RBCs/ aggregate) than in the HV (29 RBC-s/aggregate, p = 0.000). We hypothesize that pathological changes during MI induce modification of the carbohydrate composition on the RBC membrane and thus modify RBC agglutination. Occurrence of carbohydrate-lectin binding sites on RBC membranes provides evidence about MI. Due to significant difference in the rate of agglutination between MI > HV the differentiation between these groups is possible based on PHA-induced RBC-agglutination. This novel assay could serve as a rapid, cost effective valuable new tool for diagnosis of MI.

Proceedings Article | 4 March 2014 Paper

Flow cytometric assay for analysis of cytotoxic effects of potential drugs on human peripheral blood leukocytes

Kathleen Nieschke, Anja Mittag, Karolina Golab, Jozsef Bocsi, Arkadiusz Pierzchalski, Wojciech Kamysz, Attila Tarnok

Proceedings Volume 8947, 89470W (2014) https://doi.org/10.1117/12.2037741

KEYWORDS: Blood, Toxicity, Statistical analysis, Capillaries, Flow cytometry, Biological research, Organisms, Silver, Veins, Chemistry

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Proceedings Article | 22 March 2013 Paper

Laser scanning cytometry as a tool for biomarker validation

Anja Mittag, Christiane Füldner, Jörg Lehmann, Attila Tarnok

Proceedings Volume 8572, 85720O (2013) https://doi.org/10.1117/12.2002339

KEYWORDS: Tissues, Luminescence, Statistical analysis, In vivo imaging, Biological research, Laser scanners, Proteins, Molecules, Animal model studies, Positron emission tomography

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Proceedings Article | 9 February 2012 Paper

Comparison of quantitative flow cytometric data provided by panels with lower and increased color number

József Bocsi, Anja Mittag, Arkadiusz Pierzchalski, Adolf Baumgartner, Ingo Dähnert, Attila Tárnok

Proceedings Volume 8225, 822512 (2012) https://doi.org/10.1117/12.906712

KEYWORDS: Luminescence, Blood, Heart, Biological research, Calibration, Algorithm development, Surgery, Flow cytometry, Fluorescence resonance energy transfer, Statistical analysis

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Proceedings Article | 23 February 2011 Paper

Classification and discrimination of pediatric patients undergoing open heart surgery with and without methylprednisolone treatment by cytomics

Jozsef Bocsi, Anja Mittag, Arkadiusz Pierzchalski, Pavel Osmancik, Ingo Dähnert, Attila Tárnok

Proceedings Volume 7902, 79021O (2011) https://doi.org/10.1117/12.876487

KEYWORDS: Surgery, Heart, Blood, Statistical analysis, Modulation, Luminescence, Biological research, Flow cytometry, Cardiology, Analytical research

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Proceedings Article | 11 February 2011 Open Access

The influence of selected antimicrobial peptides on the physiology of the immune system

Karolina Golab, Anja Mittag, Arkadiusz Pierzchalski, Jozsef Bocsi, Wojciech Kamysz, Attila Tarnok

Proceedings Volume 7902, 790214 (2011) https://doi.org/10.1117/12.876485

KEYWORDS: Amplifiers, Flow cytometry, Bacteria, Physiology, Pathogens, Therapeutic agents, Defense and security, Tissues, Resistance, Antimicrobial agents

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Proceedings Article | 25 February 2010 Paper

Label-free single cell analysis with a chip-based impedance flow cytometer

Arkadiusz Pierzchalski, Monika Hebeisen, Anja Mittag, Marco Di Berardino, Attila Tarnok

Proceedings Volume 7568, 75681B (2010) https://doi.org/10.1117/12.840865

KEYWORDS: Cell death, Opacity, Capacitance, Electrodes, Tissues, Physiology, Microfluidics, Dielectric spectroscopy, Flow cytometry, Plasma

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Proceedings Article | 13 February 2009 Paper

Evaluation of optimal DNA staining for triggering by scanning fluorescence microscopy (SFM)

Anja Mittag, Monika Marecka, Arkadiusz Pierzchalski, Wolf Malkusch, József Bocsi, Attila Tárnok

Proceedings Volume 7182, 71820L (2009) https://doi.org/10.1117/12.808920

KEYWORDS: Luminescence, Atomic force microscopy, Signal detection, Optical filters, Statistical analysis, Microscopes, Blood, Biological research, Microscopy, Signal analyzers

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Proceedings Article | 29 February 2008 Paper

Immunological changes following protein losing enteropathy after surgery total cavopulmonary connection (TCPC) by cytomics

József Bocsi, Dominik Lenz, Anja Mittag, Ursula Sauer, Lena Wild, John Hess, Dietmar Schranz, Jörg Hambsch, Peter Schneider, Attila Tárnok

Proceedings Volume 6859, 68590N (2008) https://doi.org/10.1117/12.761640

KEYWORDS: Surgery, Proteins, Blood, Heart, Luminescence, Cardiology, Receptors, Analytical research, Biological research, Cardiovascular surgery

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Proceedings Article | 19 February 2007 Paper

Quantitative tissue cytometry (Tissomics): multimodal slide-based cytometry, confocal imaging, and volume rendering is the key

Attila Tarnok, Anja Mittag, Jens-Peer Kuska, Ulf-Dietrich Braumann, Birgit Mosch, Thomas Arendt

Proceedings Volume 6441, 64410H (2007) https://doi.org/10.1117/12.698530

KEYWORDS: Tissues, Confocal microscopy, Luminescence, Brain, Biological research, Image analysis, Neuroimaging, Microscopy, Bioinformatics, Laser scanners

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Proceedings Article | 6 February 2007 Paper

Hyperchromatic laser scanning cytometry

Attila Tárnok, Anja Mittag

Proceedings Volume 6430, 643011 (2007) https://doi.org/10.1117/12.698597

KEYWORDS: Luminescence, Biological research, Analytical research, Fluorescence resonance energy transfer, Laser scanners, Statistical analysis, Neodymium, Microscopes, Biology, Molecules

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Proceedings Article | 27 March 2006 Paper

Automated four color CD4/CD8 analysis of leukocytes by scanning fluorescence microscopy using Quantum dots

Jozsef Bocsi, Anja Mittag, Viktor Varga, Bela Molnar, Zsolt Tulassay, Ulrich Sack, Dominik Lenz, Attila Tarnok

Proceedings Volume 6096, 60960Z (2006) https://doi.org/10.1117/12.644544

KEYWORDS: Luminescence, Atomic force microscopy, Quantum dots, Microscopes, Blood, Biological research, Microscopy, Optical filters, Multiplexing, Analytical research

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Proceedings Article | 21 February 2006 Paper

Clinical cytomics

Attila Tárnok, Anja Mittag, Dominik Lenz

Proceedings Volume 6088, 60880K (2006) https://doi.org/10.1117/12.645024

KEYWORDS: Medicine, Tissues, Biological research, Proteins, Data acquisition, Surgery, Flow cytometry, Cancer, Heart, Multiplexing

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Proceedings Article | 1 April 2005 Paper

Small and cheap: accurate differential blood count with minimal sample volume by laser scanning cytometry (LSC)

Anja Mittag, Dominik Lenz, Paul Smith, Susanne Pach, Attila Tarnok

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.588653

KEYWORDS: Blood, Luminescence, Diagnostics, Laser scanners, Statistical analysis, Argon ion lasers, Cardiology, Heart, Microscopes, Signal detection

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Aim: In patients, e.g. with congenital heart diseases, a differential blood count is needed for diagnosis. To this end by standard automatic analyzers 500 μl of blood is required from the patients. In case of newborns and infants this is a substantial volume, especially after operations associated with blood loss. Therefore, aim of this study was to develop a method to determine a differential blood picture with a substantially reduced specimen volume. Methods: To generate a differential blood picture 10 μl EDTA blood were mixed with 10 μl of a DRAQ5 solution (500μM, Biostatus) and 10 μl of an antibody mixture (CD45-FITC, CD14-PE, diluted with PBS). 20 μl of this cell suspension was filled into a Neubauer counting chamber. Due to the defined volume of the chamber it is possible to determine the cell count per volume. The trigger for leukocyte counting was set on DRAQ5 signal in order to be able to distinguish nucleated white blood cells from erythrocytes. Different leukocyte subsets could be distinguished due to the used fluorescence labeled antibodies. For erythrocyte counting cell suspension was diluted another 150 times. 20 μl of this dilution was analyzed in a microchamber by LSC with trigger set on forward scatter signal. Results: This method allows a substantial decrease of blood sample volume for generation of a differential blood picture (10 μl instead of 500μl). There was a high correlation between our method and the results of routine laboratory (r²=0.96, p<0.0001; n=40). For all parameters intra-assay variance was less than 7 %. Conclusions: In patients with low blood volume such as neonates and in critically ill infants every effort has to be taken to reduce the blood volume needed for diagnostics. With this method only 2% of standard sample volume is needed to generate a differential blood picture. Costs are below that of routine laboratory. We suggest this method to be established in paediatric cardiology for routine diagnostics and for resource poor settings.

Proceedings Article | 29 March 2005 Paper

Immunophenotyping by slide-based cytometry and by flow cytometry are comparable

Andreas Gerstner, Wiebke Laffers, Anja Mittag, Ingo Daehnert, Domnik Lenz, Friedrich Bootz, Jozsef Bocsi, Attila Tarnok

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.588644

KEYWORDS: Luminescence, Flow cytometry, Blood, Glasses, Laser scanners, Statistical analysis, Signal detection, Cardiology, Laser systems engineering, Monoclonal antibodies

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Proceedings Article | 24 March 2005 Paper

Tissomics: two- and three-dimensional distribution of nuclei in brain tissue using laser scanning cytometry (LSC)

Domnik Lenz, Anja Mittag, Birgit Mosch, Jozsef Bocsi, Thomas Arendt, Attila Tarnok

Proceedings Volume 5701, (2005) https://doi.org/10.1117/12.590695

KEYWORDS: Tissues, Brain, Luminescence, Solids, Neurons, Laser tissue interaction, Neuroimaging, Laser scanners, Argon ion lasers, Data acquisition

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Proceedings Article | 1 July 2004 Paper

Sequential photobleaching for increasing the measurable fluorochromes by laser scanning cytometry

Anja Mittag, Dominik Lenz, Jozsef Bocsi, Ulrich Sack, Andreas Gerstner, Attila Tarnok

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.528208

KEYWORDS: Luminescence, Optical filters, Blood, Laser scanners, Statistical analysis, Data acquisition, Glasses, Biological research, Fluorescence resonance energy transfer, Microscopes

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For immunophenotypic analysis more measurable parameters for the discrimination of leukocyte subsets are necessary. With a single scan six fluorochromes can be distinguished with the Laser Scanning Cytometer (LSC). Due to the number of PMTs the amount of simultaneously measurable fluorescences per scan is limited. Nevertheless, the amount of measurable colors can be improved to eight by appropriate change of the filter settings and two scans per specimen. Aim of this study was to use the special features of Slide based Cytometry (SBC) beyond filter change, remeasurement and merging to distinguish fluorochromes with similar emission spectra. The photosensitivity of fluorochromes that are excited and emit in a similar wavelength range may be very different. The number of measurable parameters per PMT was increased using photosensitivity of different fluorochromes as additional criteria. Peripheral blood leukocytes were stained with antibodies conjugated to the fluorochromes APC, APC-Cy5.5 and Alexa-Fluor 633 and mounted on conventional uncoated glass slides with Fluorescence mounting medium. Specimens were excited in the LSC with the HeNe (633nm) Laser and measured at different filter settings (670/20nm-filter for APC/ALEXA 633 and 710/20nm-filter for APC-Cy5.5). At this point, APC-Cy5.5 and APC/ALEXA633 were already distinguishable. In order to differentiate between APC and ALEXA633 photobleaching was performed by repeated excitation with the laser at 633nm. Control measurements proved that APC is much more sensitive against laser excitation, i.e. looses much more fluorescence intensity than ALEXA633. The separate measurements (before/after filter change and before/after bleaching) were merged into one file. The photostability of Alexa-Fluor 633 (1.02% bleach per scan) and APC (5.74% bleach per scan) are substantially different. Therefore, after bleaching and merging both fluorochromes can be distinguished and are regarded by the software as separate parameters. The fluorochromes APC/ALEXA633 and APC-Cy5.5 can be discriminated by changing the emission filters before bleach. By sequential photobleaching, change of filters and subsequent merging of the data the number of simultaneously measurable “colors” is substantially increased.

Proceedings Article | 22 June 2004 Paper

Clinical and laboratory applications of slide-based cytometry with the LSC, SFM, and the iCYTE imaging cytometer instruments

Jozsef Bocsi, Ed Luther, Anja Mittag, Ingo Jensen, Ulrich Sack, Dominik Lenz, Lajos Trezl, Viktor Varga, Beea Molnar, Attila Tarnok

Proceedings Volume 5328, (2004) https://doi.org/10.1117/12.529314

KEYWORDS: Atomic force microscopy, Cell death, Luminescence, Microscopes, Microscopy, Tumors, Digital imaging, Optical filters, Signal detection, Blood

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Showing 5 of 18 publications

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